Research Interests
The research in my lab is aimed at expanding our basic understanding of the molecular mechanisms controlling
programmed cell death (PCD) in plants. We have developed a pathogen-free model
system based on the response of Arabidopsis
thaliana to a purified fungal toxin,
fumonisin B1 (FB1).
Two complementary strategies are being taken: 1) an Arabidopsis leaf mesophyll
protoplast system to quantitatively examine PCD in defense-related mutants and
to characterize the signal transduction pathway(s) controlling FB1-induced PCD;
and 2) a genetic approach to identify Arabidopsis mutants resistant to
FB1-induced PCD and/or affected in expression of ethylene- and
jasmonate-dependent defense-related genes.
FB1 causes formation of necrotic lesions on Arabidopsis that resemble plant
pathogen-induced lesions in many respects, including deposition of phenolic
compounds and callose, accumulation of camalexin, production of reactive oxygen
intermediates, and induction of pathogenesis-related (PR) gene expression. FB1-induced cell death in protoplasts is
dependent on de novo transcription, translation, and reversible protein
phosphorylation, confirming that FB1 elicits PCD in Arabidopsis. Roles for salicylic acid, ethylene and
jasmonate in mediating FB1-induced PCD is supported by evaluation of
FB1-induced cell death in defense-related Arabidopsis mutants and transgenic
plants. To identify other factors
contributing to FB1-induced PCD, Arabidopsis FB1-resistant (fbr) mutants were selected directly
by sowing seeds on FB1-containing agar media.Further characterization of fbr mutants confirms that this high throughput mutant selection
can reveal mutants with pathogen phenotypes. Moreover, the selection may also be useful in identifying components of
developmental pathways.
Recent Papers
Sadaf Khan and Julie M. Stone (2007) Arabidopsis thaliana GH3.9 influences primary root growth. Planta (DOI 10.1007/s00425-006-0462-2).
Laurence V. Bindschedler, Julia Dewdney, Kris A. Blee, Julie M. Stone, Tsuneaki Asai, Julia Plotnikov, Carine Denoux, Tezni Hayes, Chris Gerrish, Dewi R. Davies, Frederick M. Ausubel, and G. Paul Bolwell (2006) Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance. The Plant Journal 47:851-863.
Tsanko Gechev, Frank Van Breusegem, Julie M. Stone, Ilya Denev and Christophe Laloi (2006) Reactive oxygen species as signals controlling plant stress responses and programmed cell death. Bioessays 28(11): 1091-1101 (Invited and peer-reviewed).
Julie M. Stone and Mark A. Wilson (2006) Other antioxidants. In Redox Biochemistry, R. Banerjee, ed., John Wiley & Sons, Inc., New York, NY. (In Press).
Julie M. Stone, Xinwen Liang, Emily R. Nekl and Justin J. Stiers (2005) Arabidopsis AtSPL14, a plant-specific SBP-domain transcription factor, participates in plant development and sensitivity to fumonisin B1. The Plant Journal 41:744-754. Journal Series No. 14541
A. Leonardo Iniguez, Yuemei Dong, Heather D. Carter, Brian M.M. Ahmer, Julie M. Stone and Eric W. Triplett (2005) Regulation of enteric endophytic bacterial colonization by plant defenses. Molecular Plant-Microbe Interactions 18(2):169-178 Journal Series No. 14428
Emily J.H. Ross, Julie M. Stone, Christian G. Elowsky, Raul Arredondo-Peter, Robert V. Klucas and Gautam Sarath (2004) Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, ARR1. Journal of Experimental Botany 55:1721-1731. Journal Series No. 13844
Wim Verelst, Jyoti Kapila, Janice de Almeida Engler, Julie M. Stone, Roland Caubergs and Han Asard (2004) Tissue-specific expression and developmental regulation of cytochrome b561 genes in Arabidopsis thaliana and Raphanus sativus. Physiologia Plantarum 120:312-318.
Julie M. Stone*, Jacqueline E. Heard*, Tsuneaki
Asai and Frederick M. Ausubel (2000) Simulation of fungal-mediated cell death
and selection of Arabidopsis fumonisin B1-resistant (fbr) mutants. The Plant Cell, 12(10): 1811-1822.
Tsuneaki Asai, Julie M. Stone*, Jacqueline E. Heard, Yelena Kovtun, Peter Yorgey, Jen Sheen and
Frederick M. Ausubel (2000) Fumonisin B1-induced cell death in Arabidopsis
protoplasts requires salicylate-, jasmonate- and ethylene- signaling
pathways. The Plant Cell, 12(10): 1823-1835.
Julie M. Stone, Tsuneaki Asai, Jacqueline E.
Heard and Frederick M. Ausubel (2000) Investigating the mechanisms of
programmed cell death in plants with a toxin from a necrotrophic fungus. In Biology of Plant-Microbe
Interactions, volume 2, P.J.G.M. de Wit, T. Bisseling and W.J. Stiekema, eds.,
APS Press, St. Paul, MN, 411-415.
Tsung-Luo Jinn, Julie M. Stone and John C. Walker (2000) Haesa, an Arabidopsis leucine rich repeat receptor kinase, controls
floral organ abscission. Genes & Development 14:108-117.
Julie M. Stone (1999) Phage-based expression
cloning to identify interacting proteins. In Current Protocols in Protein Science, F.M. Ausubel, R. Brent, R.E. Kingston,
D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, eds., John Wiley &
Sons, Inc., New York, NY, pp. 19.3.1-19.3.9.
Julie M. Stone and John C. Walker (1999)
Interaction cloning of protein kinase partners. In Protein Phosphorylation: A Practical Approach, 2nd edition, D.G.
Hardie ed., Oxford University Press, Oxford, UK, pp. 281-290.
Julie M. Stone*, Amy E. Trotochaud*, John C.
Walker and Steven E. Clark (1998) Control of meristem development by CLAVATA1
receptor kinase and KAPP protein phosphatase interactions. Plant
Physiology 117: 1217-1225.
Attila L. Ádám, Sharon Pike, Mary E. Hoyos, Julie M. Stone, John C. Walker and
Anton Novacky (1997) Rapid and transient activation of a myelin basic protein
kinase in tobacco leaves treated with harpin from Erwinia amylovora. Plant Physiology 115: 853-861.
David M. Braun, Julie M. Stone and John C. Walker (1997) Interaction of the maize and Arabidopsis
kinase interaction domains with a subset of receptor-like protein kinases:
implications for transmembrane signaling in plants.Plant Journal 12: 83-95.
Julie M. Stone, Margaret A. Collinge, Robert D.
Smith, Mark A. Horn and John C. Walker (1994) Interaction of a protein
phosphatase with an Arabidopsis
serine/threonine receptor kinase. Science 266: 793-795.
Conferences
Poster: “Redox regulation of the Arabidopsis thaliana transcription factor, FBR6, involved in programmed cell death” Emily J.H. Ross, Xinwen Liang & Julie M. Stone. South Dakota Plant Physiology/Plant Biochemistry Symposium “From Plants to Genes and Back Again”. Brookings, SD August 6-8, 2003
Poster: “Positional cloning of Arabidopsis thaliana programmed cell death mutants” Justin J. Stiers and Julie M. Stone. South Dakota Plant Physiology/Plant Biochemistry Symposium “From Plants to Genes and Back Again”. Brookings, SD August 6-8, 2003
Oral: “Redox-Regulation of the A. thaliana Programmed Cell Death-Related Transcription Factor, FBR6” Emily J. H. Ross and Julie M. Stone EMBO Practical Course Fellow: Plant Development: Molecular and Cellular Basis. (2003) April 29 - March 17, Oeiras, Portugal.
Poster: Programmed Cell Death in Plant Development: Characterization of a Transcriptional Regulator, Julie M. Stone, Xinwen Liang, Justin Stiers, Sadaf Khan and Emily Ross. Keystone Symposium,”Plant biology: Functions and control of cell death”, Snowbird, UT April 10-16, 2003.
Poster: UNL Undergraduate Research Symposium – Spring 2003, “Positional Cloning of an Arabidopsis thaliana Mutant Resistant to Fumonisin B1” Justin Stiers and Julie Stone.
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