Research Interests
My research interests
are in developing tools for functional genomics and implementing genetic
engineering for plant germplasm enhancement targeting value added and disease
control traits. The laboratory has research programs on soybean, wheat, maize,
sorghum, and turf grasses. I serve as
the director of the Plant Transformation Core Research Facility (PTCRF) at the
University of Nebraska. The PTCRF is a
stateofart facility that provides services for vector constructions, plant
transformations and characterizations of the derived transformants.
Recent Papers
Clemente, T., B.J. LaValle, A.R. Howe, D.C. Ward, R.J. Rozman, P.E. Hunter, D.L.
Broyles, D.S. Kasten, M.A. Hinchee. 2000. Progeny analysis of glyphosate selected
transgenic soybeans derived from Agrobacterium-mediated transformation. Crop Sci.
40:797-803.
Campbell, B.T., P.S. Baenziger, A. Mitra, S. Sato, T. Clemente. 2000. Inheritance of
multiple transgenes in wheat. Crop Sci. 40:1133-1141.
Xing, A., S. Sato, P. Staswick, Z. Zhang, T. Clemente. 2000 The use of the two T-DNA
binary system to obtain marker-free transgenic soybeans. Cell. Dev. Biol. 36:456-463
Clemente, T.E., D. Shah, M. Tran, D. Stark, S. Padgette, D. Dennis, K. Brükner, A.
Steinbüchel, T. Mitsky. 2000. Sequence of PHA synthase gene from two strains of
Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across
two heterologous expression systems. Appl. Microbiol. Biotechnol. 53:420-429.
Rodriguez, R.M., C. Haseltine, K. Huess-LaRossa, T. Clemente, J. Soto, P. Staswick,
P. Blum. 2000. Autohydrolysis of plant polysaccharides using transgenic
hyperthermophilic enzymes. Biotechnology and Bioengineering 70:151-159
Luo, Z,Q., T.E. Clemente, S.K. Farrand. 2001. Construction of an Agrobacterium C58
derivative that does not mutate to tetracycline resistance. Mol. Plant. Micro. Int.
14:98-03.
Dickman, M.B., Y.K. Park, T. Oltersdorf, T. Clemente, and R. French. 2001.
Abrogation of disease development in plants expressing animal antiapoptotic genes.
Proc. Natl. Acad. Sci. USA. 98:6957-6962.
Farrand, S, T. Clemente and P. Staswick 2001. Counter selection strategy for
gram negative bacteria U.S. Patent Application.
Clemente, T., T. Buhr and P. Staswick. 2001. Down-regulation of single genes and
simultaneous down-regulation of multiple genes by nuclear localization of RNA
transcripts. U.S. Patent Application
Staswick, P. Z, Zhang, T. Clemente, and J. Specht. 2001. Efficient down regulation of
the major vegetative storage protein genes in transgenic soybean does not compromise
plant productivity. Plant Physiol. 127:1819-1826.
Fei, S., T. Yu, T. Clemente, and T. Riordan. 2001. Nodal segment explant as a potential
target for the genetic engineering of buffalograss. Int. Turgrass Soc. Res. J. 9:165-168.
Lee, T-J, D. Coyne, T. Clemente, and A. Mitra. 2002. Partial resistance to bacterial wilt
in transgenic tomato plants expressing non-plant antibacterial lactoferrin gene.
J. Amer. Soc. Hort. Sci. 127:158-164.
Buhr, T, S. Sato, F. Ebrahim, A. Xing, Y. Zhou, M. Mathiesen, B. Schweiger, A.
Kinney, P. Staswick, T. Clemente. 2002. Ribozyme termination of RNA transcripts
down-regulate seed fatty acid genes in transgenic soybean. Plant J. 30:155-163.
Ebmeier, A., L. Allison, H. Cerutti, and T. Clemente. 2004 Evaluation of the Escherichia
coli threonine deaminase gene as a selectable marker for plant transformation.
Planta 218:751-758.
Sato, S., T. Clemente, and I. Dweikat. 2003. Identification of an elite sorghum
genotype with high in vitro performance capacity. In Vitro Cell. Dev. Biol.-
Plant 40:57-60.
Sato, S., A. Xing, X. Ye, B. Schweiger, A. Kinney, G. Graef, and T. Clemente. 2004.
Production of δ-linolenic acid and stearidonic acid in seeds of marker-free transgenic
soybean. Crop Sci. 44:646-652.
Piller, K., T. Clemente, S. M. Jun, C. C. Petty, S. Sato, D. W. Pascual, and K. L. Bost.
2004. Expression and immunogenicity of an E. coli K99 fimbriae subunit in soybean.
Planta. In press.
Jones, R. W., M. Ospina-Giraldo, and T. Clemente. 2004. Prosystemin-antimicrobial-
peptide fusion reduces tomato late blight lesion expansion Mol. Breeding 14:83-89.
Kinney, A. J. and T. Clemente 2005. Modifying soybean oil for enhanced
performance in biodiesel blends. Fuel Process. Technol. 86:1137-1147.
Piller, K., T. Clemente, S. M. Jun, C. C. Petty, S. Sato, D. W. Pascual, and K. L.
Bost. 2005. Expression and immunogenicity of an E. coli K99 fimbriae subunit
in soybean. Planta. 222:6-18.
Vasconcelos, M., H. Eckert, V. Arahana, G. Graef, M. A. Grusak, and T.
Clemente. 2006. Molecular and phenotypic characterization of transgenic
soybean expressing the Arabidopsis ferric chelate reductase gene, FRO2.
Planta. 224:1116-1128.
Eckert, H., B. LaVallee, B. J. Schweiger, A. J. Kinney, E. B. Cahoon, and T.
Clemente. 2006. Co-expression of the borage ?6 desaturase and the
Arabidopsis ?15 desaturase results in high accumulation of stearidonic acid in
the seeds of transgenic soybean. Planta. 224:1050-1057
Clemente, T. Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana). 2006
Methods Mol. Biol. 343:143-154.
Qu, F., X. Ye, G. Hou, S. Sato, T. E. Clemente, and T. J. Morris. 2005 RDR6
has a broad-spectrum but temperature-dependent antiviral defense role in
Nicotiana benthamiana. J. Virol. 79:15209-15217.
Sato, S., A. Xing, X. Ye, B. Schweiger, A. Kinney, G. Graef, and T. Clemente.
2004. Production of ?-linolenic acid and stearidonic acid in seeds of marker-
free transgenic soybean. Crop Sci. 44:646-652
Ebmeier, A., L. Allison, H. Cerutti, and T. Clemente. 2004 Evaluation of the
Escherichia coli threonine deaminase gene as a selectable marker for plant
transformation. Planta 218:751-758.
Howe, A., S. Sato, I. Dweikat, M. Fromm, and T. Clemente. 2006. Rapid and
reproducible Agrobacterium-mediated transformation of sorghum. Plant Cell
Rep. 25:784-791
Garg, R., M. Tolbert, J.L. Oakes, T. E. Clemente, K.L. Bost, and K.J. Piller. 2007. Chloroplast targeting of FanC, the major antigenic subunit of Escherichia coli K99 fimbriae, in transgenic soybean. Plant Cell Rep. In press
Xu, W., S.J. Sato, T.E. Clemente, and R. Chollet. 2007. The PEP-carboxylase kinase gene family in Glycine max (GmPpcK1-4): an in-depth molecular analysis with nodulated, non-transgenic and transgenic plants. Plant J. 49:910-923.
Behrens, M. R., N. Mutlu, S. Chakraborty, R. Dumitru, W.-H. Jiang, B. J. LaVallee, P. L. Herman, T. E. Clemente and D. P. Weeks. 2007 Dicamba resistance: Enlarging and preserving biotechnology-based weed management strategies. Science 316:1185-1188.
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